Journal: Cancers

Article Title: BRCA1 Promoter Hypermethylation is Associated with Good Prognosis and Chemosensitivity in Triple-Negative Breast Cancer

doi: 10.3390/cancers12040828

Figure Lengend Snippet: Correlation between methylation-specific PCR and EpiTYPER® MassARRAY® methylation evaluation ( n = 153). Black columns indicate discordant cases ( n = 4, methylation percentage assessed by MassARRAY® = 9.5, 6, 4, and 4), using a positivity cut-off of 10%.

Article Snippet: The key secondary endpoint was the evaluation of the concordance between the determination of BRCA1 promoter methylation status by the quantitative EpiTyper ® MassARRAY ® (Agena Bioscience, Sans Diego, CA, USA) and by MS-PCR.

Techniques: Methylation

Journal: Cancers

Article Title: BRCA1 Promoter Hypermethylation is Associated with Good Prognosis and Chemosensitivity in Triple-Negative Breast Cancer

doi: 10.3390/cancers12040828

Figure Lengend Snippet: Univariate clinicopathological correlations with BRCA1 promoter methylation using MS-PCR.

Article Snippet: The key secondary endpoint was the evaluation of the concordance between the determination of BRCA1 promoter methylation status by the quantitative EpiTyper ® MassARRAY ® (Agena Bioscience, Sans Diego, CA, USA) and by MS-PCR.

Techniques: Methylation, Expressing

Journal: Cancers

Article Title: BRCA1 Promoter Hypermethylation is Associated with Good Prognosis and Chemosensitivity in Triple-Negative Breast Cancer

doi: 10.3390/cancers12040828

Figure Lengend Snippet: Distribution of CpG sites analyzed in the promoter region of the BRCA1 gene. ( A ) The BRCA1–NBR2 locus: the position of the first exons of the BRCA1 is indicated by shaded boxes. The first exon of NBR2 gene is indicated by a black box. ( B ) Position of CpG sites analyzed Cytosine on CpG sites tested by MS-PCR are underlined. Cytosine on CpG sites tested by MassARRAY® EpiTYPER® assay are in bold. The positive strand of the BRCA1 gene is shown based on GenBank accession number U37574. The transcription start site of the BRCA1 exon 1A is marked by an arrow. Exon 1A sequence is in capital, and both 5’upstream sequence and intron 1 sequence are in lower case characters.

Article Snippet: The key secondary endpoint was the evaluation of the concordance between the determination of BRCA1 promoter methylation status by the quantitative EpiTyper ® MassARRAY ® (Agena Bioscience, Sans Diego, CA, USA) and by MS-PCR.

Techniques: MassARRAY EpiTYPER assay, Sequencing